Major Equipment - Advanced light Microscopy and Spectroscopy
1. PicoQuant MicroTime 200, confocal time-resolved microscope with diffraction limited spatial resolution, single molecule sensitivity and sub-nanosecond time resolution
- Four detection channels based on single photon avalanche (SPAD) detectors
- Multicolor excitation modalities (pulsed-interlieved excitation, pulse burst excitation)
- Available excitation wavelenghts: 375, 405, 440, 470, 532, 640 nm
- 3D objective scanning using XY piezo stage with 10 nm moving precision and objective Z-positioner
- Wide field fluorescence observation using mercury lamp and CCD camera
2. Home built confocal time-resolved microscope with diffraction limited spatial resolution, single molecule sensitivity and sub-nanosecond time resolution
- Two detection channels based on single photon avalanche (SPAD) detectors
- Multicolor excitation modalities (pulsed-interlieved excitation, pulse burst excitation)
- Available excitation wavelenghts: 375, 405, 440, 470, 532, 640 nm
- 3D objective scanning using XYZ piezo stage with 10 nm moving precision
3. Olympus FluoView1000 MPE, confocal laser scanning microscope with multi-photon as well as single photon excitation capability
- Three built-in PMT detectors
- Two channel, non-descanned detection unit based on PMT detectors with sub-nanosecond TCSPC (FLIM) capability
- Two channel confocal detection unit based on single photon avalanche (SPAD) detectors with sub-nanosecond TCSPC (FLIM) and FCS capability
- Transmitted light detector
- Wide field fluorescence observation using mercury lamp and color CCD camera
- Coherent Chameleon, tunable Ti Sapphire laser for multiphoton excitation
- Available conventional excitation wavelenghts: 470, 488, 532, 640 nm
4. Home built single molecule sensitive fluorescence microscope with TIRF objective based on Olympus IX71 frame and Andor DU897_BV EMCCD camera
- wide-field epifluorescence time-lapse imaging with TIRF capability
- Image splitter for dual color (or dual plane or astigmatic) observation
- Single molecule localisation microscopy (e.g. PALM and SOFI) capability
- TOCCSL (Thinning Out Clusters while Conserving Stoichiometry of Labeling) capability
- Coherent Sapphire (488 and 560 nm) an Coherent Cube (405 and 640 nm) CW excitation sources
- Combined micro (XY) and piezo-nano (XYZ) positioner from MadCityLabs
5. Home built TIRF microscope targeted at camera based Imaging FCS and Single Particle Tracking based on Olympus IX81 frame and Andor iXon Ultra 897 BV EMCCD camera
- TOCCSL (Thinning Out Clusters while Conserving Stoichiometry of Labeling) and Single Particle Tracking (SPT) capability
- Coherent Sapphire 488 nm and Lasos 640 nm CW excitation sources
- Image splitter for dual color (or dual plane or astigmatic) observation
6. IBH model 5000U Time Resolved Spectrofluorimeter
- Based on Time-Correlated Single-Photon Counting (TCSPC)
- Hamamatsu MCP detector
- Broad selection of laser diode and pulsed LED excitation sources
- Excitation and emission polarization accessories
- Temperature controlled sample holder
- Intensity decay, anisotropy decay, TRES measurement capabilities
1. PicoQuant MicroTime 200, confocal time-resolved microscope with diffraction limited spatial resolution, single molecule sensitivity and sub-nanosecond time resolution
- Four detection channels based on single photon avalanche (SPAD) detectors
- Multicolor excitation modalities (pulsed-interlieved excitation, pulse burst excitation)
- Available excitation wavelenghts: 375, 405, 440, 470, 532, 640 nm
- 3D objective scanning using XY piezo stage with 10 nm moving precision and objective Z-positioner
- Wide field fluorescence observation using mercury lamp and CCD camera
2. Home built confocal time-resolved microscope with diffraction limited spatial resolution, single molecule sensitivity and sub-nanosecond time resolution
- Two detection channels based on single photon avalanche (SPAD) detectors
- Multicolor excitation modalities (pulsed-interlieved excitation, pulse burst excitation)
- Available excitation wavelenghts: 375, 405, 440, 470, 532, 640 nm
- 3D objective scanning using XYZ piezo stage with 10 nm moving precision
3. Olympus FluoView1000 MPE, confocal laser scanning microscope with multi-photon as well as single photon excitation capability
- Three built-in PMT detectors
- Two channel, non-descanned detection unit based on PMT detectors with sub-nanosecond TCSPC (FLIM) capability
- Two channel confocal detection unit based on single photon avalanche (SPAD) detectors with sub-nanosecond TCSPC (FLIM) and FCS capability
- Transmitted light detector
- Wide field fluorescence observation using mercury lamp and color CCD camera
- Coherent Chameleon, tunable Ti Sapphire laser for multiphoton excitation
- Available conventional excitation wavelenghts: 470, 488, 532, 640 nm
4. Home built single molecule sensitive fluorescence microscope with TIRF objective based on Olympus IX71 frame and Andor DU897_BV EMCCD camera
- wide-field epifluorescence time-lapse imaging with TIRF capability
- Image splitter for dual color (or dual plane or astigmatic) observation
- Single molecule localisation microscopy (e.g. PALM and SOFI) capability
- TOCCSL (Thinning Out Clusters while Conserving Stoichiometry of Labeling) capability
- Coherent Sapphire (488 and 560 nm) an Coherent Cube (405 and 640 nm) CW excitation sources
- Combined micro (XY) and piezo-nano (XYZ) positioner from MadCityLabs
5. Home built TIRF microscope targeted at camera based Imaging FCS and Single Particle Tracking based on Olympus IX81 frame and Andor iXon Ultra 897 BV EMCCD camera
- TOCCSL (Thinning Out Clusters while Conserving Stoichiometry of Labeling) and Single Particle Tracking (SPT) capability
- Coherent Sapphire 488 nm and Lasos 640 nm CW excitation sources
- Image splitter for dual color (or dual plane or astigmatic) observation
6. IBH model 5000U Time Resolved Spectrofluorimeter
- Based on Time-Correlated Single-Photon Counting (TCSPC)
- Hamamatsu MCP detector
- Broad selection of laser diode and pulsed LED excitation sources
- Excitation and emission polarization accessories
- Temperature controlled sample holder
- Intensity decay, anisotropy decay, TRES measurement capabilities